Coupling stimuli-responsive magnetic nanoparticles with antibody-antigen detection in immunoassays.
نویسندگان
چکیده
Because current homogeneous immunoassays show some limitations, particularly low sensitivity, we developed a new immunoassay to overcome these limitations. The approach was based on magnetic nanoparticles with a thermoresponsive polymer layer, a negatively charged polymer, and streptavidin-biotin-based antibody-antigen detection and yielded higher sensitivity than commonly used heterogeneous immunoassays. Because no special equipment is needed, it can be applied to currently available absorbance-based systems for high-throughput assays.
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ورودعنوان ژورنال:
- Analytical chemistry
دوره 83 24 شماره
صفحات -
تاریخ انتشار 2011